Anti-HEK293 HCP Antibody

Reactivity HCPs from HEK293, CAP,
Hela, A549, PER.C6, etc.
Coverage >96%
Detection >2500 HMW & LMW HCPs
Applications 1D/2D WB, ELISA, IP
Host Species Goat

Shipping Info: Costs calculated at checkout. FedEx / UPS Overnight. Currently shipping only to USA.

Category:

ProductCatalog No.TypeSizePrice
Anti-HEK293 HCP Antibody: SerumHEK-S101Serum100 µl$379
HEK-S102Serum500 µl$1269
Anti-HEK293 HCP Antibody: IgGHEK-G101IgG0.5 mg$695
HEK-G102IgG1.0 mg$999

Anti-HEK293 HCP Antibody

  • Reactivity: Host cell proteins from HEK293, CAP, Hela, A549, PER.C6, etc.
  • Coverage: > 96%
  • Detection: HMW and LMW host cell proteins
  • Applications: 1D/2D Western Blot, ELISA & Immunoprecipitation (IP)
  • Type: Serum or IgG
  • Host Species: Goat

Product Details:

  • Specificity: Detect both cellular and culture media (CM) high molecular weight (HMW) and low molecular weight (LMW) proteins from human cell lines such as HEK293, CAP, Hela, A549, PER.C6, etc.
  • Immunogen: High molecular weight (HMW) and low molecular weight (LMW) HEK293 proteins
  • Purity: Antiserum or IgG. This antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
  • Cross-Reactivity: Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Applications:

  • Applications: 1D/2D Western Blot, ELISA, IP
  • Application notes: Anti-HEK293 HCP antibody detects more than 2500 HEK293 total proteins with antibody coverage > 97% by 2D Western Blot. The antibody coverage in each quadrant is also > 93.2%.
  • Suggested dilutions: 1:1000 ~ 1:5000 for Western Blot, 1:2000 ~ 1:10000 for ELISA. Optimized dilutions may vary depending on testing samples and applications.

Formulation:

  • Concentration: ~75 mg/mL (serum antibody) by Bradford Protein Assay
  • Buffer: 20 mM Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.4
  • Preservative: 0.01% (w/v) Sodium Azide
  • Stabilizer: None

Handling

  • Precaution of use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
  • Storage condition: Store at 4°C if using within 1-2 weeks. Upon delivery, aliquot and freeze at -20°C for extended storage. Avoid freeze / thaw cycles. Before using, bring to room temperature and centrifuge product if not completely clear.

Our Anti-HEK293 HCP antibody offers high sensitivity detection and maximum coverage in the following applications:

A. HEK293 HCP antibody coverage by 2D Western Blot

150 µg HCP from HEK293 was labeled with Cy3 and run on large format 2D gel. After SDS-PAGE, the HCP image was scanned (top left), followed by transferring to membrane. The membrane was incubated with Anti-HEK293 HCP Antibody (HEK-S101) at a 1:2000 dilution at 4oC overnight. Cy5-labeled Donkey anti-Goat antibody was used for detection (top middle). Both HEK293 HCP (green) and WB (red) were scanned from the same membrane to generate the overlay image (top right).

HEK HCP 2D Gel

Anti-HEK HCP 2D WB

HEK HCP / Western Blot

HEK HCP spot map

Anti-HEK HCP WB spot map

Quantitation of antibody coverage: Anti-HEK293 HCP Antibody

  • Anti-HEK293 HCP antibody coverage: 98.7%
    • Brown: 3078 spots detected by Anti-HEK HCP Antibody
    • Blue: 95 spots not detected by Anti-HEK HCP Antibody

  • Quadrant coverage
    • Quadrant 1: 99.4%
    • Quadrant 2: 99.6%
    • Quadrant 3: 93.2%
    • Quadrant 4: 93.8%

B. HEK293 HCP antibody coverage by 1D Western Blot

20 µg HCP from HEK293 was labeled with Cy3 and run on a 1D gel. After SDS-PAGE, HCP image was scanned, followed by transferring to membrane. The membrane was incubated with Anti-HEK293 HCP antibody (HEK-G101) at 8 µg/mL dilution at 4oC overnight. Cy5 labeled Donkey anti-Goat antibody was used for detection. Both HEK293 HCP (green) and WB (red) were scanned from the same membrane to generate the overlay image.

C. ELISA

The following data was generated using HEK-S101:

  • Coating: HEK293-HCP protein in the indicated amount in 100 µl of PBS. 
  • Primary antibody: Anti-HEK293 HCP Antibody (HEK-S101) at 1:2000 dilution
  • Secondary antibody: HRP conjugated anti-goat IgG at 1: 5000 dilution
  • Substrate: TMB for 20 minutes.
Graph of ELISA data for Anti-HEK293 HCP Antibody

Precautions:

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Disclaimer:

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Applied Biomics are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Applied Biomics, Inc.

Type

Serum – 100 µl, Serum – 500 µl, IgG – 0.5 mg, IgG – 1.0 mg